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Item Tag: Daily Bio

N-(1-Naphthyl)-ethylenediamine dihydrochloride 10g

Product Description:N-(1-Naphthyl)-ethylenediamine, dihydrochlorideTotal Product Size:10gIndividual Container Size:10gNumber of Containers:1Shipping Conditions:RTUNSPSC Code:12352200UNSPSC Category:Biochemical Reagents

Cell culture Dishes Tissue culture treated, 35X10mm, PS, Sterile 500 per case

Product Description:EZ-LINE Cell culture Dishes Tissue culture treated, 35X10mm, PS, Sterile TC treated, 500 per case: Cell culture dishes for tissue culture work. All dishes are produced with optically clear, high quality crystalline polymers. Growth surface is treated for optimal cell attachment and growth and designed for reduced cross-contamination.Total Product Size:500UNITIndividual Container Size:500UNITNumber of Containers:1Shipping Conditions:RTUNSPSC Code:41122107UNSPSC Category:Tissue Culture Dishes

Peptone C 100G PEPTONIZED MILK B927.SIZE.100G

Product Description:Peptone C 100G PEPTONIZED MILK OverviewPeptone is obtained by hydrolyzing proteins such as blood fiber with pepsin or other enzymes. Soluble in water, insoluble in ethanol, chloroform and ether. ApplicationIt is used as the main raw material for microorganism and animal cell culture medium. Size: 100 grams Shipping Conditions:RTUNSPSC Code:41106200UNSPSC Category:Microbiological Media

Coomassie brilliant blue G-250 100G

Product Description:Coomassie brilliant blue G-250Number of Containers:1Shipping Conditions:RTUNSPSC Code:12171500UNSPSC Category:Dyes and Stains

HEPES, free acid 250g

Product Description:HEPES, free acid: HEPES has been described as one of the best all-purpose buffers available for biological research. At most biological pHs the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. Buffer strength for cell culture applications is usually in the range of 10 to 25 mM. After the addition of HEPES pH is adusted with NaOH or HCl. Care must be taken to maintain appropriate osmolality in media, and toxicity with respect to a given cell line must be evaluated. (Isotonicity data have been tabulated.) HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. HEPES is not recommended for certain protein applications; it interferes with the Folin-Ciocalteu protein assay. The Biuret protein assay is unaffected. HEPES was the buffer of choice in a protein deposition technique in electron microscopy because it did not affect metal substrates. HEPES was evaluated and shown to be quite suitable for use with Ampholines in generating pH gradients less than 1 pH unit wide for isoelectric focusing applications. A buffer solution of HEPES can be prepared by any of several methods. The free acid can be added to water, then titrated with approximately one-half mole equivalent of sodium hydroxide or potassium hydroxide to the pH desired, a simple mixing table for preparing 0.05 M HEPES/NaOH has been published. Alternatively, equimolar concentrations of HEPES and of sodium HEPES can be mixed in approximately equal volumes, back-titrating with either solution to the appropriate pH. Shipping Conditions:RTUNSPSC Code:12161700UNSPSC Category:Buffers

HEPES, free acid 100g

Product Description:HEPES, free acid: HEPES has been described as one of the best all-purpose buffers available for biological research. At most biological pHs the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. Buffer strength for cell culture applications is usually in the range of 10 to 25 mM. After the addition of HEPES pH is adusted with NaOH or HCl. Care must be taken to maintain appropriate osmolality in media, and toxicity with respect to a given cell line must be evaluated. (Isotonicity data have been tabulated.) HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. HEPES is not recommended for certain protein applications; it interferes with the Folin-Ciocalteu protein assay. The Biuret protein assay is unaffected. HEPES was the buffer of choice in a protein deposition technique in electron microscopy because it did not affect metal substrates. HEPES was evaluated and shown to be quite suitable for use with Ampholines in generating pH gradients less than 1 pH unit wide for isoelectric focusing applications. A buffer solution of HEPES can be prepared by any of several methods. The free acid can be added to water, then titrated with approximately one-half mole equivalent of sodium hydroxide or potassium hydroxide to the pH desired, a simple mixing table for preparing 0.05 M HEPES/NaOH has been published. Alternatively, equimolar concentrations of HEPES and of sodium HEPES can be mixed in approximately equal volumes, back-titrating with either solution to the appropriate pH. Shipping Conditions:RTUNSPSC Code:12161700UNSPSC Category:Buffers

HEPES, free acid 50g

Product Description:HEPES, free acid: HEPES has been described as one of the best all-purpose buffers available for biological research. At most biological pHs the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. Buffer strength for cell culture applications is usually in the range of 10 to 25 mM. After the addition of HEPES pH is adusted with NaOH or HCl. Care must be taken to maintain appropriate osmolality in media, and toxicity with respect to a given cell line must be evaluated. (Isotonicity data have been tabulated.) HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. HEPES is not recommended for certain protein applications; it interferes with the Folin-Ciocalteu protein assay. The Biuret protein assay is unaffected. HEPES was the buffer of choice in a protein deposition technique in electron microscopy because it did not affect metal substrates. HEPES was evaluated and shown to be quite suitable for use with Ampholines in generating pH gradients less than 1 pH unit wide for isoelectric focusing applications. A buffer solution of HEPES can be prepared by any of several methods. The free acid can be added to water, then titrated with approximately one-half mole equivalent of sodium hydroxide or potassium hydroxide to the pH desired, a simple mixing table for preparing 0.05 M HEPES/NaOH has been published. Alternatively, equimolar concentrations of HEPES and of sodium HEPES can be mixed in approximately equal volumes, back-titrating with either solution to the appropriate pH. Shipping Conditions:RTUNSPSC Code:12161700UNSPSC Category:Buffers